Part:BBa_K1698002:Experience

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Applications of BBa_K1698002

The Sry 55bp detector was tested with single stranded, double stranded and double stranded targets with background DNA present in UCC_Ireland iGEM 2014 team.

Results for Sry detector reacted with an oligonucleotide target.

Number of colonies

No oligo 0 cfu

ssSry target oligo 125 cfu

The detector had yet to be reacted with genomic DNA. This was initially carried out by Srijita Banarjee as a final year research project, and again repeated by Cork iGEM 2015 using the detector constructed by the new PCR amplification protocol [http://http://2015.igem.org/Team:Cork_Ireland/Project]

Figure Legend Sry detector reacted with HaeIII digested male and female genomic DNA, as well as uncut genomic DNA and a negative control with no target DNA.

User Reviews

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